MAB4146

Anti-BCRP Antibody, clone BXP-21

culture supernatant, clone BXP-21, Chemicon^®

• 동의어(들): Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: BXP-21, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 입력: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• 동형: IgG2a
• NCBI 수납 번호: NM_004827.2
• UniProt 수납 번호: Q9UNQ0
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ABCG2(9429)

설명

일반 설명

The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates

특이성

This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.

면역원

Fusion protein consisting of the E. coli maltose-binding protein and a 126-aa portion of BCRP (aa 271-396).

애플리케이션

Immunocytochemistry:
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.

Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

This Anti-BCRP Antibody, clone BXP-21 is validated for use in IC, IH, IH(P), WB for the detection of BCRP.

품질

Evaluated by Western Blot on HepG2 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.

표적 설명

70 kDa

물리적 형태

Mouse monoclonal IgG2a in buffer containing 0.7% BSA and 0.1% sodium azide

Unpurified

저장 및 안정성

For long term storage, store at -20ºC. Stable for 6 months from date of receipt at 2-8ºC.

분석 메모

Control
MCF7 cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2