Detects a cation- and ligand-induced binding site (LIBS) of integrin beta 1, which has been used in some systems as a reporter of integrin functionality.
애플리케이션
Immunoprecipitation of detergent-solubilized (1 integrins: 2-4 μg/IP
Western blot: 10-15 μg/mL
Flow Cytometry: 10-15 μg/mL
Immunohistochemistry
Stimulation of (1-mediated adhesion: 10-20 μg/mL.
Optimal working dilutions must be determined by end user.
This Anti-Integrin β1 Antibody, activated, clone B44, azide free is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.
표적 설명
88 kDa
물리적 형태
Format: Purified
Purified immunoglobulin. Liquid PBS, containing no preservatives.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
American journal of physiology. Renal physiology, 307(6), F695-F707 (2014-07-25)
To explore whether matrix stiffness affects cell differentiation, proliferation, and transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in primary cultures of mouse proximal tubular epithelial cells (mPTECs), we used a soft matrix made from monomeric collagen type I-coated polyacrylamide gel
European journal of immunology, 31(12), 3504-3512 (2001-12-18)
Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major
Matrix biology : journal of the International Society for Matrix Biology, 41, 19-25 (2014-12-03)
Osteopontin (OPN) is a ligand for the α4ß1 integrin, but the physiological importance of this binding is not well understood. Here, we have assessed the effect of post-translational modifications on OPN binding to the α4 integrin on cultured human leukocyte
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 41(11), 2723-2732 (2016-06-09)
Estradiol (E2) perfusion rapidly increases the strength of fast excitatory transmission and facilitates long-term potentiation in the hippocampus, two effects likely related to its memory-enhancing properties. Past studies showed that E2's facilitation of transmission involves activation of RhoA signaling leading
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