FCMAB193F

Milli-Mark^® Anti-CD33-FITC Antibody, clone WM-54

clone WM-54, Milli-Mark^®, from mouse

• 동의어(들): Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: FITC conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: WM-54, monoclonal
• 종 반응성: human
• 제조업체/상표: Milli-Mark^®
• 기술: flow cytometry: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_001763.3
• UniProt 수납 번호: P20138
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD33(945)

설명

일반 설명

CD33 is a member of the siglec family (sialic acid binding immunoglobulin-like lectins) referred to as Siglec-3 (Freeman SD, Kelm S, Barber EK and Crocker PR, 1995, Blood, 2005-2012). It is the smallest member of the siglec family having a molecular weight of 67 kDa (Peiper SC, Ashmun RA and Look TA (1988) Blood., 72: 314-321). CD33 mediates protein-carbohydrate cellular interactions in addition to protein-protein recognition (Crocker, 2002). The CD33 gene is located on chromosome 19 (Peiper, 1997). CD33 is expressed by subsets of monocytes and neutrophils. CD33 is the major antigen to differentially diagnose myeloid from lymphoid leukemias, as it is expressed on 90% of myeloid and not lymphoid leukemic cells (Andrews 1989; Griffin 1984; and Sabbath 1986). Committed hemapoietic progenitors, including multipotential stem cells (CFU-GEMM) and precursors of granulocytes and macrophages (CFU-GM) and early erythroid progenitors (BFU-E) express CD33 (Peiper, 1989 and 1997). Anti-CD33, WM-54, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD33 (Peiper, 1989).

특이성

Antibody recognizes Human CD33 antigen.

면역원

Leukemic cells from a patient with AML.

애플리케이션

Milli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

품질

Evaluated by flow cytometry using human PBMCs

표적 설명

67 kDa (Peiper, 1997)

물리적 형태

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

저장 및 안정성

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

분석 메모

Control
PBMCs

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable