For cellular staining using SSEA-1 in flow cytometry, prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. Dispense the volume per test of the working solution according to the number of cells indicated below:
- Use 5 µL for Guava Flow Cytometer with 5x10^5 cells in 95 µL PBS, totaling 100 µL.
- Use 10 µL for other flow cytometry systems with 1x10^6 cells in 90 µL PBS, totaling 100 µL.
추천 제품
생물학적 소스
mouse
Quality Level
결합
PE
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
MC-480, monoclonal
종 반응성
rat, mouse, human
기술
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
동형
IgM
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... FUT4(2526)
관련 카테고리
일반 설명
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
특이성
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
면역원
Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).
애플리케이션
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
품질
Evaluated by Flow Cytometry with mouse embryonic stem cells.
표적 설명
220 kDa Calculated
물리적 형태
Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.
저장 및 안정성
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
분석 메모
Control
Mouse embryonic Stem Cells
Mouse embryonic Stem Cells
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells.
Anchan, RM; Lachke, SA; Gerami-Naini, B; Lindsey, J; Ng, N; Naber, C; Nickerson et al.
Testing null
Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice.
Chang, BS; Choi, YJ; Kim, JH
Journal of Reproduction and Development null
Lei Zhai et al.
PloS one, 12(2), e0172420-e0172420 (2017-02-18)
Embryonic stem cells (ESCs) are pluripotent cells and have the capability for differentiation into any of the three embryonic germ layers. The Wnt/β-Catenin pathway has been shown to play an essential role in ESC differentiation regulation. Activation of β-Catenin by
Xinyu Liu et al.
Cell division, 15(1), 12-12 (2020-12-10)
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) has opened new therapeutic possibilities. However, karyotypic abnormalities detected in iPSCs compromised their utility, especially chromosomal aberrations found at early passages raised serious safety concerns. The mechanism underlying the chromosomal abnormality
-
Suggest suitable dilaution of SSEA-1 for flow cutometry.
1 답변-
도움이 되었습니까?
-
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
