콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

AG105

Sigma-Aldrich

Black Gold II Myelin Staining Kit

동의어(들):

Myelin stain kit

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12161503
eCl@ss:
32161000
NACRES:
NA.41

제조업체/상표

BLACK-GOLD

기술

immunohistochemistry: suitable

배송 상태

ambient

일반 설명

Black-Gold II is an aurohalophosphate complex which stains specifically for myelin within the central nervous system. Black-Gold II staining provides high resolution, high contrast, a short histochemical processing time, and high reproducibility. Black-Gold II is a new and improved version of its predecessor, Black-Gold (Cat # AG390). The advantages of Millipore’s Black-Gold II Myelin Staining Kit over Black Gold I are that it is more readily soluble, can be utilized at a higher concentration, produces a more uniform and consistent staining, takes less time to stain, and does not require post-staining intensification procedures.

The use of Black-Gold II is tailored to studies using formalin-fixed, non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while the individual myelinated fibers appear black. This novel tracer can be used to localize both normal and pathological myelin. Black-Gold II can demonstrate and characterize specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, methamphetamine, acrylamide, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid.

The Black-Gold II Myelin Staining Kit also comes with sodium thiosulfate and cresyl violet. The sodium thiosulfate solution is required for extracting excess Black-Gold II stain. Whereas the cresyl violet is a Nissl stain that colors cell bodies a brilliant bluish hue and is used as a counter stain with Black-Gold II.


Black Gold II Purity: No detectable amount of uncomplexed gold was found.

Black Gold II Illumination: Bright field or dark field.

Black Gold II Solubility: Freely soluble in water, saline, or dilute acids.

성분

Black-Gold II: 150mg dry powder, yellow in color.

Cresyl Violet Stain: 150mg dry powder, purplish-black in color.

5% Sodium Thiosulfate: 10ml

물리적 형태

See Components

저장 및 안정성

The kit can be stored at room temperature. Ideally, the Black-Gold II powder should be kept in a desiccator, because of its hygroscopic nature. The dry powder, if unopened and stored properly, is useable for up to one year from date of receipt. Once resuspended the Black-Gold II staining solution should be stored in the dark at 4°C and is good for up to 3 months.

TOXICITY: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

L Schmued et al.
Brain research, 837(1-2), 289-297 (1999-08-06)
A novel haloaurophosphate complex called Black-Gold has been synthesized and applied to localize myelin within the central nervous system. The technique is tailored to studies using formalin fixed non-solvent processed tissue. The technique stains large myelinated tracts dark red-brown, while
Introducing Black-Gold II, a highly soluble gold phosphate complex with several unique advantages for the histochemical localization of myelin.
Schmued, Larry, et al.
Brain Research, 1229, 210-217 (2008)
Aibo Wang et al.
Aging and disease, 10(5), 937-948 (2019-10-09)
In searching for the drainage route of the interstitial fluid (ISF) in the deep brain, we discovered a regionalized ISF drainage system as well as a new function of myelin in regulating the drainage. The traced ISF from the caudate
J Marc Simard et al.
Translational stroke research, 3(Suppl 1), 155-165 (2012-06-19)
Subarachnoid hemorrhage (SAH) can lead to disabling motor, cognitive, and neuropsychological abnormalities. Part of the secondary injury to cerebral tissues associated with SAH is attributable to the neuroinflammatory response induced by blood. Heparin is a pleiotropic compound that reduces inflammatory
Ian Tagge et al.
PloS one, 11(4), e0152480-e0152480 (2016-04-08)
Cuprizone administration in mice provides a reproducible model of demyelination and spontaneous remyelination, and has been useful in understanding important aspects of human disease, including multiple sclerosis. In this study, we apply high spatial resolution quantitative MRI techniques to establish

질문

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.