ABF210

Anti-MDA5 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): MDA5 Antibody, Melanoma differentiation-associated protein 5, Interferon-induced with helicase C domain protein 1, IFIH1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt 수납 번호: Q9BYX4
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IFIH1(64135)

설명

일반 설명

Melanoma differentiation-associated protein 5 (MDA5), also known as Interferon-induced helicase C domain-containing protein 1 (IFIH1) is a member of the helicase family (RLR subfamily). MDA5 plays an important role in recognizing various viral components and triggers antiviral responses. When stimulated by dsRNA, MDA5 recruits adaptor protein VISA causing the activation of IRF-3 and NF-κB. MDA5 is widely expressed at low levels with barely detectable levels found in , testis and lung. Mutations in MDA5 have been associated with diabetes mellitus insulin-dependent type 19.

특이성

This antibody recognizes the C-terminus of MDA5.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human MDA5.

애플리케이션

Detect Melanoma differentiation-associated protein 5 using this rabbit polyclonal antibody, Anti-MDA5 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected MDA5 in human lymph node tissue lysate.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected MDA5 in human lymph node cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

품질

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MDA5 in 15 µg of Daudi cell lysate.

표적 설명

~117 kDa observed. Uncharacterized bands may appear in some lysates.

결합

Replaces: MABF198

물리적 형태

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Daudi cell Lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable