ABF1168

Anti-Chitinase-1 (CHIT1)

from rabbit, purified by affinity chromatography

• 동의어(들): Chitotriosidase-1, EC: 3.2.1.14, Chitinase-1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_003456.1
• UniProt 수납 번호: Q13231
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CHIT1(1118)

설명

일반 설명

Chitotriosidase-1 (UniProt: Q13231; also known as EC: 3.2.1.14, Chitinase-1) is encoded by the CHIT1 gene (Gene ID: 1118) in human. Chitinase-1 is a member of the Chitinase class II subfamily that degrades chitin, chitotriose and chitobiose. It is one of the most abundantly secreted protein and is mainly produced by activated macrophages and epithelial cells. Chitinase -1 can hydrolyze the beta-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. It may also participate in the defense against nematodes and other pathogens. Four isoforms of Chitinase-1 have been described that are produced by alternative splicing and isoform 3, which lacks amino acids 344-372, does not possess any enzymatic activity. Chitinase-1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved to generate the mature form. Its chitin-binding domain is located in amino acids 417-466 and has three chitooligosaccharide binding regions (aa 7-71; 97-100; and 210-213). Elevated levels of Chitinase-1 are observed in cases of Gaucher′s disease, bronchial asthma, and atherosclerosis. (Ref.: Kanneganti, M., et al. (2012). J. Epithel. Biol. Pharmacol. 5; 1-9).

특이성

This rabbit polyclonal antibody detects Chitinase-1 in human serum. It targets an epitope within 15 amino acids from the C-terminal region.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Chitinase-1.

애플리케이션

Anti-Chitinase-1 (CHIT1), Cat. No. ABF1168, is a rabbit polyclonal that detects Chitinase-1 in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Chitinase-1 (CHIT1) in human spleen and bone marrow tissue sections.

Research Category
Inflammation & Immunology

품질

Evaluated by Western Blotting in human serum.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Chitinase-1 in human serum.

표적 설명

~ 52 kDa observed. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable