ABE1957

Anti-CENP-C Antibody

serum, from rabbit

• 동의어(들): Centromere protein C, CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_001803
• UniProt 수납 번호: Q03188
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CENPC(1060)

설명

일반 설명

Centromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to cause mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.

특이성

This rabbit polyclonal antiserum targets the N-terminal region of human CENP-C present in both spliced isoforms reported by UniProt (Q03188). Specificity was demonstrated by a reducion of target band detection (Western blotting) and kinetochores staining (chromsome spreads fluoresence staining) following cellular CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703).

면역원

Epitope: N-terminus

GST-tagged recombinant human CENP-C N-terminal fragment.

애플리케이션

Anti-CENP-C Antibody, Cat. No. ABE1957, is a highly specific rabbit polyclonal antibody that targets CENP-C and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:10,000 dilution from a representative lot immunostained kinetochores in chromosome spreads prepared from interphase DLD-1 human colorectal adenocarcinoma cells hypotonically swollen and fixed with 4% formaldehyde.

Immunocytochemistry Analysis: A representative lot was affinity purified and detected a time-dependent loss of kinetochores CENP-C immunoreactivity in 4% formaldehyde-fixed, 0.5% Triton X-100-permeabilized HeLa cells following CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703).

Immunocytochemistry Analysis: A representative lot was affinity purified and immunostained kinetochores by indirect fluorescence staining of chromosome spreads prepared from patient-derived, PD-NC4 chromosome variant harboring fibroblasts hypotonically swollen and fixed with 4% formaldehyde (Bassett, E.A., et al. (2010). J. Cell Biol. 190(2):177-185).

Western Blotting Analysis: A representative lot was affinity purified and detected a time-dependent CENP-C level in HeLa cells following CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703).

Research Category
Epigenetics & Nuclear Function

품질

Evaluated by Western Blotting in DLD-1 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected CENP-C in 50,000 cell equivalent of DLD-1 human colorectal adenocarcinoma cell lysate.

표적 설명

~140 kDa observed. 106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated. The larger-than-calculated target band size is consistent with that reported in the literature (Earnshaw, W.C., et al. (1989). Chromosoma. 98(1):1-12). Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable