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Merck
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주요 문서

P2921

Sigma-Aldrich

Monoclonal Anti-Protein A antibody produced in mouse

clone SPA-27, ascites fluid

동의어(들):

Monoclonal Anti-Protein A

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크기 선택

100 μL
₩910,151
0.2 ML
₩1,177,197

₩910,151


구입 가능 여부는 고객센터에 문의하십시오.

벌크 견적 요청

크기 선택

보기 변경
100 μL
₩910,151
0.2 ML
₩1,177,197

About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

₩910,151


구입 가능 여부는 고객센터에 문의하십시오.

벌크 견적 요청

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

SPA-27, monoclonal

포함

15 mM sodium azide

기술

immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

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일반 설명

Protein A is a 42 kDa single chain polypeptide isolated from the cell wall of Staphylococcus aureus Cowan I strain.

특이성

Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

면역원

protein A from Cowan I strain of Staphy­lococcus aureus.

애플리케이션

Monoclonal Anti-Protein A antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in western blot and dot blot analyses.

생화학적/생리학적 작용

Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used in these methods either in its native form or conjugated to various markers.

물리적 형태

Monoclonal Anti-Protein A antibody produced in mouse is provided as ascites fluid with 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Comparison of protein A affinity sorbents III. Life time study
Hahn R, et al.
Journal of Chromatography A, 1102(1-2), 224-231 (2006)
Pernille Koefoed-Nielsen et al.
Journal of clinical apheresis, 32(3), 163-169 (2016-06-04)
Transplanting immunized patients requires immunological monitoring in the pretransplant phase to follow reduction of donor specific HLA antibodies (DSA) after Staphylococcus aureus protein A (SPA) immunoadsorption (IA) or therapeutic plasma exchange followed by IVIG and Rituximab administration. Pretreatment aims to
Matthew B Frankel et al.
Molecular microbiology, 78(1), 238-252 (2010-10-07)
The human pathogen Staphylococcus aureus requires cell wall anchored surface proteins to cause disease. During cell division, surface proteins with YSIRK signal peptides are secreted into the cross-wall, a layer of newly synthesized peptidoglycan between separating daughter cells. The molecular
Stephanie Marroquin et al.
Infection and immunity, 87(5) (2019-03-06)
Numerous factors have, to date, been identified as playing a role in the regulation of Agr activity in Staphylococcus aureus, including transcription factors, antisense RNAs, and host elements. Herein we investigated the product of SAUSA300_1984 (termed MroQ), a transmembrane Abi-domain/M79
Jasdeep S Nanra et al.
Human vaccines & immunotherapeutics, 9(3), 480-487 (2012-12-20)
Staphylococcus aureus can cause severe life threatening invasive diseases. The principal immune effector mechanism by which humans are protected from Gram positive bacteria such as S. aureus is antigen specific antibody- and complement-dependent opsonophagocytosis. This process can be measured in

질문

  1. I would like to know the concentration (mg/mL) of the anti-proA antibody. Thanks!

    1 답변
    1. The concentration of this antibody is reported on a lot specific basis. The product specification range is 6 - 12 mg/mL. A brief review of the lot history shows a typical range of 8 - 10 mg/mL. Please see the link below to review a sample or lot specific certificate:
      https://www.sigmaaldrich.com/product/sigma/p2921#product-documentation

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