제품 AB3265은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.
추천 제품
제품명
Anti-Actin γ Antibody, serum, Chemicon®
생물학적 소스
sheep
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human, rat
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ACTG1(71)
일반 설명
Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html}
특이성
Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis.
면역원
Epitope: gamma
Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH.
애플리케이션
Anti-Actin Antibody, γ is an antibody against Actin for use in IC, IH & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Western Blot: 1:500-1:2,000 or greater; non-specific bands can occur when primary antibody is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kDa band. Specificity for gamma actin determined by 2D gel electrophoresis.
Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol.
Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol.
Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used.
Optimal working dilutions must be determined by the end user.
표적 설명
42-43kDa
물리적 형태
Serum. Liquid, containing no preservatives.
Unpurified
저장 및 안정성
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Smooth muscle
Smooth muscle
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Zengdun Shi et al.
Laboratory investigation; a journal of technical methods and pathology, 97(12), 1412-1426 (2017-10-17)
Liver fibrosis, a model wound healing system, is characterized by excessive deposition of extracellular matrix (ECM) in the liver. Although many fibrogenic cell types may express ECM, the hepatic stellate cell (HSC) is currently considered to be the major effector.
Don C Rockey et al.
The American journal of pathology, 189(11), 2209-2220 (2019-09-03)
In the liver, smooth muscle α-actin (SM α-actin) is up-regulated in hepatic stellate cells (HSCs) as they transition to myofibroblasts during liver injury and the wound healing response. Whether SM α-actin has specific functional effects on cellular effectors of fibrosis
S D Burton et al.
Neuroscience, 215, 1-16 (2012-05-01)
Neuroligins are a family of cell adhesion molecules critical in establishing proper central nervous system connectivity; disruption of neuroligin signaling in vivo precipitates a broad range of cognitive deficits. Despite considerable recent progress, the specific synaptic function of neuroligin-1 (NL1)
Chandrashekara Kyathanahalli et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(45), 14090-14095 (2015-10-28)
We previously identified myometrial caspase-3 (CASP3) as a potential regulator of uterine quiescence. We also determined that during pregnancy, the functional activation of uterine CASP3 is likely governed by an integrated endoplasmic reticulum stress response (ERSR) and is consequently limited
Zengdun Shi et al.
American journal of physiology. Gastrointestinal and liver physiology, 318(3), G504-G517 (2020-01-14)
Activation of hepatic stellate cells (HSCs), characterized by development of a robust actin cytoskeleton and expression of abundant extracellular matrix (ECM) proteins, such as type 1 collagen (COL.1), is a central cellular and molecular event in liver fibrosis. It has
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
