70856

RNase A Solution

Convenient solution for selective degradation of RNA

• 동의어(들): RNase A Enzyme
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 형태: solution
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze
• 농도: 10 mg/mL
• 기술: DNA purification: suitable
• 배송 상태: ambient
• 저장 온도: 10-30°C

설명

일반 설명

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

애플리케이션

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
• to treat control adult and embryonic thymi cells during staining
• to remove RNA from affibody–mRNA fusion clones before clone screening

생화학적/생리학적 작용

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.

경고

Toxicity: Standard Handling (A)

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2