5.08940

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested

• 동의어(들): G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested, G418, G 418
• 실험식(Hill 표기법): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• CAS Number: 108321-42-2
• Molecular Weight: 692.71
• MDL number: MFCD00058314
• UNSPSC 코드: 12352200

속성

• 분석: ≥98% (TLC)
• Quality Level: 200
• 양식: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 저장 온도: −20°C
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• InChI key: UHEPSJJJMTWUCP-NKCAIAFTSA-N

설명

일반 설명

Recommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

포장

Packaged under inert gas

경고

Toxicity: Harmful (C)

물리적 형태

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.

재구성

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

기타 정보

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany