A1720

G 418 disulfate salt

powder, non-animal origin, suitable for cell culture, BioReagent

• 동의어(들): Antibiotic G418
• 실험식(Hill 표기법): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• CAS Number: 108321-42-2
• Molecular Weight: 692.71
• MDL number: MFCD00058314
• UNSPSC 코드: 12352207
• PubChem Substance ID: 24890581
• NACRES: NA.76

속성

• 제품명: G 418 disulfate salt, powder, BioReagent, suitable for cell culture
• 제품 라인: BioReagent
• Quality Level: 200
• 양식: powder
• 광학 활성: [α]/D (Specific Rotation: +104.4^o (c=0.3% in H₂O at 26^oC))
• 효능: ≥720 μg per mg (Dried basis)
• 분자량: 692.7
• 포장: pkg of 1 g; pkg of 25 g; pkg of 5 g
• 기술: cell culture | mammalian: suitable
• 색상: white to off-white
• solubility: H₂O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
• 항생제 활성 스펙트럼: Gram-negative bacteria
• 동작 모드: protein synthesis | interferes
• 저장 온도: 2-8°C
• SMILES string: OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
• InChI key: UHEPSJJJMTWUCP-NKCAIAFTSA-N

설명

일반 설명

Chemical structure: aminoglycoside

G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes.[1] It is commonly known as geneticin selective antibiotic.

애플리케이션

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells[2][3][4] transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

생화학적/생리학적 작용

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.[5]

주의사항

The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

제조 메모

G418 powder is soluble in water at 100 mg/mL.

기타 정보

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)