324717

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

• 동의어(들): Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endo-β-N-acetylglucosaminidase H, Endo H
• CAS Number: 52769-51-4
• 효소 위원회 번호: 3.2.1.96 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 결합: (N-linked)
• 형태: liquid
• 특이 활성도: ≥3 units/mL; ≥30 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• 외래 활성: N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Note: 1 mU = 1 milliunit.

Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.

물리적 형태

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

기타 정보

Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable