324699

Elastase Substrate IV, Colorimetric

A specific substrate for pancreatic elastase.

• 동의어(들): Elastase Substrate IV, Colorimetric
• 실험식(Hill 표기법): C₂₅H₃₄N₆O₉
• Molecular Weight: 562.57
• UNSPSC 코드: 12352200
• NACRES: NA.54

속성

• Quality Level: 100
• 분석: ≥95% (HPLC)
• 형태: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles; desiccated
• 색상: white to off-white
• solubility: ethanol: 1 mg/mL; water: soluble
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

A specific substrate for pancreatic elastase (k_m = 100 µM; k_cat/ K_m = 35,000 M^-1 s^-1 for rat pancreatic elastase, K_m = 30 µM; k_cat/K_m = 35,000 M^-1 s^-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
A specific substrate for pancreatic elastase

Product does not compete with ATP.

Reversible: no

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

경고

Toxicity: Standard Handling (A)

서열

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

물리적 형태

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

재구성

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

기타 정보

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable