324682

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

• 동의어(들): Elastase, Porcine Pancreas, High Purity, Crystallized
• CAS Number: 39445-21-1
• 효소 위원회 번호: 3.4.21.36 (BRENDA, IUBMB)
• MDL number: MFCD00130998
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• Quality Level: 100
• 형태: lyophilized
• 특이 활성도: ≥25 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated
• solubility: distilled water: 10 mg/mL
• 외래 활성: Chymotrypsin ≤0.2%
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α₂-macroglobulin.

Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.

물리적 형태

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

재구성

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

기타 정보

Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3