219364

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• 동의어(들): Cathepsin B, Human Liver
• CAS Number: 9047-22-7
• 효소 위원회 번호: 3.4.22.1 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: human liver
• Quality Level: 100
• 분석: >95% (SDS-PAGE)
• 형태: liquid
• 특이 활성도: ≥50 units/mg protein
• 정제법: affinity chromatography
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 기술: activity assay: suitable
• pH 범위: 3.5—6.0
• 적합성: suitable for molecular biology
• 응용 분야: life science and biopharma
• 배송 상태: wet ice
• 저장 온도: −70°C
• 유전자 정보: human ... CTSB(1508)

설명

일반 설명

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.

Optimal pH: 3.5-6.0.

애플리케이션

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.

생화학적/생리학적 작용

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

물리적 형태

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

제조 메모

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable