Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.
Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.
Recognizes the ~44 kDa CD40 protein in Raji cells.
면역원
transfected murine L cells expressing human CD40
애플리케이션
Flow cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
기타 정보
FACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Fanslow, W.C., et al. 1992. J. Immunol.149, 655. Law, C.L., et al. 1990. Leukemia4, 732.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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