14-792-M

PI 3-Kinase (p110α(H1047R)/p85α), 10 µg

Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R & untagged, recombinant, human p85α full length.

• 동의어(들): Active PI 3-Kinase Protein, PI 3-Kinase p110α/p85α
• UNSPSC 코드: 12352200
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in baculovirus infected Sf21 cells
• 제품 라인: Upstate^®
• 양식: liquid
• 특이 활성도: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 분자량: M_w 125 kDa (p110α(H1047R)); M_w 84 kDa (p85α)
• 정제법: (Ni²⁺/NTA-agarose)
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 색상: colorless
• solubility: water: soluble
• 수납 번호(accession number): U79143
• UniProt 수납 번호: P26450 (mouse); P27986; P42336 / P27986;P42337 / P26450 (mouse); P42336; P42337 (mouse); U79143
• 응용 분야: life science and biopharma
• 유전자 정보: human ... PIK3CA(5290)

설명

일반 설명

Research area: Cell Signaling

Complex of N-terminal 6His-tagged recombinant human p110α full length, containing the mutation H1047R, and untagged, recombinant, human p85α full length. The H1047R substitution is a somatic mutation in p110α that has been associated with tumours of the colon, stomach, breast, and brain. PI3Ks are categorized into three classes, namely class I, II, and III, based on their structural characteristics, substrate specificities, and the nature of lipid end-products. Class IA PI3Ks are composed of heterodimers that include a p110 catalytic subunit and a p85 regulatory subunit. In mammals, PIK3CA, PIK3CB, and PIK3CD, encode the p110 catalytic isoforms: p110α, p110β, and p110δ, respectively.[1]

생화학적/생리학적 작용

PI3 kinases play a critical role in various cellular processes such as cell survival, proliferation, differentiation, apoptosis, DNA repair, angiogenesis, cellular metabolism, and motility.[2][1] PI3Ks can activate the serine/threonine kinase AKT and other downstream effector pathways by transmitting signals from growth factors and cytokines into intracellular messages. Genetic mutation in p110α is observed in breast, pancreatic, colon, liver, and lung cancers.[1]Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and can induce oncogenic transformation.

포장

Also available in 10 μg size and in bulk

Also available in 250μg size --call for pricing and availability and reference catalog number 14-792M when ordering the 250μg size.

표적 설명

PI3Kα/p85α

물리적 형태

50 mM Tris/HCl pH8.0, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°C): Not applicable