1.25079

Fractogel^® EMD DEAE (M)

MiniChrom Fractogel^® DEAE (M), volume 5 mL, prepacked column

• 동의어(들): MiniChrom Column Fractogel^® DEAE (M), 5 ml
• UNSPSC 코드: 41115711

속성

• ligand: diethyl-(2-hydroxypropyl)aminoethyl
• Quality Level: 100
• material: polypropylene housing
• 제품 라인: Fractogel^®
• 형태: prepacked column
• 파라미터: ≤8 bar pressure; ~170 cm/hr max. flow rate
• bed L: 100 mm
• 베드 용적: 5 mL
• 컬럼 I.D.: 8 mm
• 용적: 5 mL
• 기질 활성군: Methacrylate
• 평균 입자 크기: 40-90 μm
• 용량: ~100 mg, BSA binding capacity
• 응용 분야: gene therapy; mAb; vaccine development
• 분리 기술: anion exchange
• 저장 온도: 15-25°C

설명

일반 설명

Weak cation exchange Fractogel^® EMD DEAE (M) resin in a convenient pre-packed 5 ml column

특징 및 장점

MiniChrom Column Fractogel^® DEAE (M), 5 ml enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

법적 정보

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany