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Merck
모든 사진(1)

주요 문서

12-309

Sigma-Aldrich

HeLa Nuclear Extract

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크기 선택

50 μG
₩278,516

₩278,516

예상 입고일2025년 4월 30일세부사항

벌크 견적 요청
모든 사진(1)

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50 μG
₩278,516

About This Item

UNSPSC 코드:
41105330
eCl@ss:
32011807
NACRES:
NA.41

₩278,516

예상 입고일2025년 4월 30일세부사항

벌크 견적 요청

생물학적 소스

human

Quality Level

100

양식

liquid

제조업체/상표

Upstate®

농도

2.0 mg/mL

기술

western blot: suitable

배송 상태

dry ice

일반 설명

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

세포주 설명

HeLa

품질

Routinely evaluated as a positive control for many of Upstates antibodies.

물리적 형태

RIPA sample buffer containing inhibitors. Frozen solution.

저장 및 안정성

Stable for 6 months at -70°C from date of shipment.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the
J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major

질문

1–2 / 2 질문  

  1. Is this product in vitro transcription grade? What are the components provided with this item? Can I used this item to test for human rna polymerase 2 transcription?

    1 답변

    1. This product is not tested for a specific application, such as transcription efficiency. The material is supplied as a single vial of a 50 ug/25 uL solution containing RIPA buffer and inhibitors. No other components are included. Note that unless otherwise stated this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Please see the link below to review available Certificates of Analysis:
      https://www.emdmillipore.com/product/HeLa-Nuclear-Extract,MM_NF-12-309#anchor_COA

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  2. Can I use this HeLa Nuclear Extract for deadenylase assay? specifically, Does this product contain the complex of deadenylase in addition to a variety of DNA binding proteins and transcription factors?

    1 답변

    1. This product has not been tested for use in deadenylation. There is no reason to suspect that it would not be suitable. However, the product is stored in RIPA buffer. Please check the literature for potential interference of RIPA in the adenylase assay reaction.

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