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Merck
모든 사진(4)

문서

05-1312-I

Sigma-Aldrich

Anti-Ubiquityl-Histone H2B Antibody, clone 56

clone 56, from mouse

동의어(들):

Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, H2B/al, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l)

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모든 사진(4)

About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

100

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

56, monoclonal

종 반응성

mouse, human, rat

포장

antibody small pack of 25 μg

기술

ChIP: suitable
western blot: suitable

동형

IgG2aκ

NCBI 수납 번호

NP_066406.1

UniProt 수납 번호

P62807

배송 상태

ambient

타겟 번역 후 변형

unmodified

유전자 정보

human ... HIST1H2BC(8347)

일반 설명

Histone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 8339, 8343, 8344, 8346, 8347) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Histone H2B is one of the core components of the nucleosome, the smallest subunit of chromatin. DNA base pairs are wrapped around an octamer of core histone proteins consisting of two each of Histone H2A, H2B, H3 and H34. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression. Histone H2B is ubiquitylated at Lys120 by RAD6 E2 protein in conjunction with RNF20/RNF40 and this ubiquitinylated form is found preferentially associated with the transcribed regions of highly expressed genes.

특이성

Clone 56 recognizes histone H2B when ubiquitinated on Lys120. It does not recognize H2B in its unmodified state or ubiquitin unconjugated to H2B.

면역원

A branched peptide corresponding to the ubiquitination site on human H2B.

애플리케이션

Anti-Ubiquityl-Histone H2B, clone 56 Antibody, Cat. No. 05-1312-I, is a highly specific mouse monoclonal antibody that targets Ubiquityl-Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in HeLa, NIH3T3, and PC-12 cell lysate.

Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in U2OS whole cell extract, purified histone H2A, purified H2B, and HEK293 acid extracted proteins (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquityl-Histone H2B in uH2B and H3K4me2 (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).

품질

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Ubiquityl-Histone H2B in 10 µg of PC-12 cell lysate.

표적 설명

~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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Computational and structural biotechnology journal, 21, 614-629 (2023-01-21)
The timing and location of writing and erasing of histone modifications determine gene expression programs and are tightly controlled processes. One such modification is the monoubiquitination of histone H2B (H2Bub), whose precise level during transcription elongation is dynamically regulated by
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Heading date is one of the most pivotal agronomic traits for rice (Oryza sativa) yield and adaptation. Little is known about the crosstalk between histone ubiquitination and histone methylation in rice heading date regulation. Here, we reported HISTONE MONOUBIQUITINATION 1
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Molecular and cellular biology, 40(15) (2020-05-06)
Rtf1 is a conserved RNA polymerase II (RNAPII) elongation factor that promotes cotranscriptional histone modification, RNAPII transcript elongation, and mRNA processing. Rtf1 function requires the phosphorylation of Spt5, an essential RNAPII processivity factor. Spt5 is phosphorylated within its C-terminal domain
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Psychiatric diseases are often accompanied by circadian disruptions, but the molecular underpinnings remain largely unclear. To address this, we screened genes that have been previously reported to be associated with psychiatric diseases and found that TRRAP, a gene associated with

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