추천 제품
생물학적 소스
rabbit
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
Apu2, monoclonal
종 반응성
rabbit, mouse, human, rat
기술
cell based assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... UBAP2(55833)
일반 설명
DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
특이성
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
면역원
Epitope: Lys48 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys48.
애플리케이션
Immunohistochemistry:
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.
Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).
Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.
Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).
Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin & has been validated in FC, ICC, IP, IHC, WB, PIA.
Research Category
Signaling
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
품질
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.
A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.
표적 설명
varies
물리적 형태
Format: Purified
Protein A purified
Protein A purified recombinant purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
분석 메모
Control
Lys48 polyubiquitin chains.
Lys48 polyubiquitin chains.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Bifunctional apoptosis regulator (BAR), an endoplasmic reticulum (ER)-associated E3 ubiquitin ligase, modulates BI-1 protein stability and function in ER Stress.
Rong, J; Chen, L; Toth, JI; Tcherpakov, M; Petroski, MD; Reed, JC
The Journal of Biological Chemistry null
CARMA1 controls Th2 cell-specific cytokine expression through regulating JunB and GATA3 transcription factors.
Blonska, M; Joo, D; Zweidler-McKay, PA; Zhao, Q; Lin, X
Journal of immunology (Baltimore, Md. : 1950) (1950)
Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
Guicciardi, ME; Werneburg, NW; Bronk, SF; Franke, A; Yagita, H; Thomas, G; Gores, GJ
Testing null
Unique insights into maternal mitochondrial inheritance in mice.
Luo, SM; Ge, ZJ; Wang, ZW; Jiang, ZZ; Wang, ZB; Ouyang, YC; Hou, Y; Schatten, H; Sun, QY
Proceedings of the National Academy of Sciences of the USA null
Atypical ubiquitin chains: new molecular signals. 'Protein Modifications: Beyond the Usual Suspects' review series.
Ikeda, Fumiyo and Dikic, Ivan
EMBO Reports, 9, 536-542 (2008)
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
