U5379

Anti-Ubiquitin antibody produced in rabbit

whole antiserum, lyophilized powder

• 동의어(들): Anti-Ubiquitin antibody
• MDL number: MFCD00162897
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: whole antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: lyophilized powder
• 분자량: antigen 8.6 kDa
• 종 반응성: prokaryotes, eukaryotes
• 기술: immunohistochemistry (frozen sections): suitable; western blot: 1:100 using ubiquitin purified from bovine red blood cells
• UniProt 수납 번호: P0CG47
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... UBB(7314) , UBC(7316) ; mouse ... Ubb(22187) , Ubc(22190) ; rat ... Ubb(192255) , Ubc(50522)

설명

일반 설명

Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2.

Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes.

특이성

By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.

면역원

bovine red blood cell ubiquitin.

애플리케이션

Anti-Ubiquitin antibody produced in rabbit is used in:

• immunostaining in neuropathology[1]
• immunohistochemistry
• western blotting

생화학적/생리학적 작용

Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis.

표적 설명

Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.

물리적 형태

Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.

재구성

Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)