LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18
材料
分析カラム
CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase
[A] 0.1% (v/v) formic acid in water; [B] 0.1% (v/v) formic acid in 75:25 (v/v) acetonitrile:water
flow rate
0.2 mL/min
gradient
2% B for 2 min, to 100% B in 38 min
column temp.
35 °C
detector
ESI(+) TOF, extracted ions m/z 463.1244, 433.1140, 505.137
injection
1 μL
詳細
アナリシスノート
The purpose of this study was to evaluate the change in anthocyanins levels in wild blueberries as a function of berry development. The delphinidin, putinidin, peonidin, and malvinidin base-modified components exhibited a dramatic increase in abundance as a function of berry development. The cyanidin base-modified components exhibited a much lower increase in abundance as a function of berry development, it is uncertain the significance of the rate increase. Chlorogenic acid exhibited a continuous decrease in abundance as a function of berry development. Overall, the combination of UV/Vis detection along with accurate mass TOF enabled a effective method of identification of anthocyanins from blueberry extracts. The Ascentis Express column provided rapid, sensitive detection and rugged operation.
その他情報
Berries (1.0 g) were added to 1.0 mL of 1% (v/v) formic acid in methanol. Samples were crushed in the solvent mixture and extracted (refrigerated) for 2 hours. A portion of the extracted sample was removed, centrifuged and the supernatant was collected for HPLC analysis.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany