LC/MS/MS Analysis of Testosterone in Human Serum on Ascentis® Express C18 after Extraction with BioSPME using C18 SPME LC Tips
CONDITIONS
sample/matrix
human plasma
SPME fiber
SPME LC Tips C18 (57234-U)
extraction
60 min with 1 mL sample
desorption process
30 min with 200 μL of methanol
column
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] 0.1% formic acid in water ; [B] methanol
gradient
5% B to 70% B in 3 min, then to 90% B in 0.1 min, hold 90% B for 0.9 min
flow rate
0.5 mL/min
pressure
4351 psi (300 bar)
column temp.
40 °C
detector
MS/MS, ESI(+), MRM (See Figure for transitions)
injection
10 μL
sample
10 ng/mL in methanol
詳細
アナリシスノート
Sensitive method for analysis of testosterone in human plasma.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany