LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5
CONDITIONS
column
Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)
mobile phase
[A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85)
flow rate
0.2 mL/min
pressure
1150 psi (79 bar)
column temp.
30 °C
detector
MS, ESI(+), MRM mode
injection
1 μL
sample
10 ng/mL in mobile phase
詳細
アナリシスノート
The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany