HPLC Analysis of Vitamins, Fat Soluble (A and E), on Discovery^® C8

column

Discovery C8, 15 cm × 4.6 mm I.D., 5 μm particles (59353-U)

mobile phase

[A] acetonitrile: [B] water (90:10 v/v, A:B)

flow rate

2 mL/min

detector

UV, 290 nm

injection

10 μL