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Merck

HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

材料

Standard

製品番号
詳細
価格

M8378

マルトトリオース

≥90% (HPLC)

84097

スクロース

BioUltra, Molecular Biology, ≥99.5% (HPLC)

47287-U

α-ラクトース

analytical standard

G0350500

グルコース

European Pharmacopoeia (EP) Reference Standard

X0200000

D-(+)-キシロース

European Pharmacopoeia (EP) Reference Standard

1375025

麦芽糖 一水和物

United States Pharmacopeia (USP) Reference Standard

分析カラム

製品番号
詳細
価格

581512-U

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

詳細

アナリシスノート

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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