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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85) application for HPLC

材料

分析カラム

製品番号
詳細
価格

53981-U

Ascentis® Express HILIC、2.7 μm HPLCカラム

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

標準

製品番号
詳細
価格

M8378

マルトトリオース

≥90% (HPLC)

84097

スクロース

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

α-ラクトース

analytical standard

G0350500

グルコース

European Pharmacopoeia (EP) Reference Standard

X0200000

D-(+)-キシロース

European Pharmacopoeia (EP) Reference Standard

1375025

麦芽糖 一水和物

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

詳細

アナリシスノート

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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