HPLC Analysis of E. coli Lysate on Proteomix^® SAX Columns, Particle Size Comparison

column temp.

25 °C

column

Proteomix SAX-NP10, 5 cm x 4.6 mm I.D., 10 μm particles (Z777203)

column

Proteomix SAX-NP5, 5 cm x 4.6mm I.D., 5 μm particles (Z777227)

detector

UV, 280 nm

flow rate

0.5 mL/min

gradient

0 - 100%B in 30 min

injection

10 μL

mobile phase

(A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl

sample

2.5 mg/mL