HPLCによるダンシル-バリンエナンチオマーの分析、 CHIROBIOTIC^® R

column

CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)

mobile phase

0.1 wt% ammonium trifluoroacetic acid in methanol

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 260 nm

injection

1 μL

sample

5 mg/mL in methanol