HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis^® Express RP-Amide (UV Detection)

column

Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)

mobile phase

[A] water; [B] acetonitrile

gradient

50 to 100% B in 10 min

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

10 μL

sample

50 mg/L of each in 50:50, water: acetonitrile