HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide
CONDITIONS
column
Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)
mobile phase
[A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile
gradient
25 to 75% B in 10 min; held at 75% B for 5 min
flow rate
0.5 mL/min
column temp.
35 °C
detector
UV, 340 nm
injection
10 μL
詳細
アナリシスノート
Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany