HPLC Analysis of 2-AMP and Piroxicam on Ascentis^® Express F5

column

Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 μm particles (53590-U)

mobile phase

[A] 10 mM ammonium formate, pH=3; [B] acetonitrile; (75:25, A:B)

flow rate

0.8 mL/min

pressure

1635 psi (113 bar)

column temp.

35 °C

detector

UV, 230 nm

injection

5 μL

sample

100 μg/mL Piroxicam and 10 μg/mL 2-AMP in mobile phase