GC Analysis of Pesticides in Beef Kidney on SLB^®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel^™ Que Z-Sep+, MS in SIM Mode, Fast GC Analysis

sample preparation

Dispersive (QuEChERS)

sample/matrix

10 gm homogenized beef kidney spiked at 50 ng/g with pesticides

extraction tube

Supel Que Acetate (AC) Tube (55234-U)

extraction process

add 10 mL of acetonitrile; shake for 1 minute; add contents of Supel Que acetate extraction tube (55234-U); shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes; (Sample Pretreatment: Homogenize 10 gm beef kidney and spike with pesticides at 50 ng/g.)

clean-up tube

Supel QuE Z-Sep+ tube (55296-U)

clean-up process

transfer 3 mL of the acetonitrile layer into a Supel QuE Z-Sep+ cleanup tube (55296-U); shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis

column

F.S. CAP. SLB-5MS 20 m x 0.18 mm I.D., 0.36 μm (28576-U)

oven

70 °C ( 0.5 min), 25 °C/min to 125 °C, 10 °C/min to 200 °C, 5 °C/min to 300 °C/min (1 min)

inj. temp.

programmed, 60 °C (0.28 min), 600 °C/min to 325 °C (5 min)

detector

MSD

MSD interface

325 °C

carrier gas

helium, 1 mL/min constant flow

injection

10 μL LVI, PTV solvent vent, rapid injection speed

liner

4 mm ID FocusLiner with taper

sample

Beef Kidney extract