GC Analysis of cis/trans FAME Column Performance Mix on SP^®-2560 per AOAC Method Ce 1h-05

材料

Standard

製品番号

詳細

価格

cis/trans F.A.M.E Column Performance Mix

2.5 mg/mL in dichloromethane, ampule of 1 mL

分析カラム

製品番号

詳細

価格

SP^®-2560キャピラリーGCカラム

L × I.D. 100 m × 0.25 mm, d_f 0.20 μm

CONDITIONS

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)

oven

180 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 1 mL/min

injection

1 μL, 100:1 split

sample

cis/trans FAME Column Performance Mix (40495-U)

詳細

アナリシスノート

Complex mixtures of cis/trans FAME isomers are difficult to separate. Baseline resolution may not be possible for some samples due to variation in GC instrument designs and column efficiencies. As a result, AOAC Method Ce 1h-05 recommends monitoring changes in column performance with a known FAME mixture closely matching the range of fatty acids under investigation.

The resolution factor for Peak 1 and Peak 2 of this mix (40495-U) is a good indicator of colum performance. Using one of the resolution equations listed on the Data Sheet, the resolution factor can be calculated. Resolution factors approaching 1.5 (baseline resolution) are desired.

For additional information, request Data Sheet T708019.

法的情報

SP is a registered trademark of Sigma-Aldrich Co. LLC

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GC Analysis of cis/trans FAME Column Performance Mix on SP®-2560 per AOAC Method Ce 1h-05

材料