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Nanodiamonds as Nucleating Agents for Protein Crystallization.

Langmuir : the ACS journal of surfaces and colloids (2017-06-13)
Yen-Wei Chen, Chien-Hsun Lee, Yung-Lin Wang, Tsung-Lin Li, Huan-Cheng Chang
要旨

Nanodiamond (ND) is a carbon-based nanomaterial with potential for a wide range of biological applications. One of such applications is to facilitate the nucleation of protein crystals in aqueous solution. Here, we show that NDs (nominal diameters of 30 and 100 nm) after surface oxidation in air and subsequent treatment in strong acids are useful as heterogeneous nucleating agents for protein crystallization. Tested with lysozyme, ribonuclease A, proteinase K, and catalase, the nanomaterials in either aggregate or film form are found to be able to increase the crystallization efficiency of all proteins. Particularly, for 30 nm NDs, the films with an area of ∼2 mm

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Sigma-Aldrich
リゾチーム ニワトリ卵白由来, powder or granules, ≥39,000 units/mg protein
Sigma-Aldrich
プロテイナーゼ K from Tritirachium album, lyophilized powder, ≥30 units/mg protein
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リボヌクレアーゼA ウシ膵臓由来, ≥70 Kunitz units/mg protein, lyophilized
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カタラーゼ ウシ肝臓由来, powder, suitable for cell culture, 2,000-5,000 units/mg protein
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ポリ-L-リシン 塩酸塩, mol wt >30,000