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Merck
  • Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor.

Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor.

Materials science & engineering. C, Materials for biological applications (2013-03-19)
Binh Hai Nguyen, Lam Dai Tran, Quan Phuc Do, Huy Le Nguyen, Ngoc Huan Tran, Phuc Xuan Nguyen
要旨

The selective detection of ultratrace amounts of aflatoxin M1 (AFM1) is extremely important for food safety since it is the most toxic mycotoxin class that is allowed to be present on cow milk with strictly low regulatory levels. In this work, Fe3O4 incorporated polyaniline (Fe3O4/PANi) film has been polymerized on interdigitated electrode (IDE) as sensitive film for AFM1 electrochemical biosensor. The immobilized aptamers as an affinity capture reagent and magnetic nanoparticles for signal amplification element have been employed in the sensing platform. Label-free and direct detection of the aptamer-AFM1 on Fe3O4/PANi interface were performed via electrochemical signal change, acquired by cyclic and square wave voltammetries. With a simplified strategy, this electrochemical aptasensor shows a good sensitivity to AFM1 in the range of 6-60 ng·L(-1), with the detection limit of 1.98 ng·L(-1). The results open up the path for designing cost effective aptasensors for other biomedical applications.

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製品内容

Sigma-Aldrich
酸化鉄(II,III), nanopowder, 50-100 nm particle size (SEM), 97% trace metals basis
Sigma-Aldrich
酸化鉄(II,III), powder, <5 μm, 95%
Sigma-Aldrich
酸化鉄(II,III), 99.99% trace metals basis
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酸化鉄(II,III)、磁性ナノ粒子 溶液, 10 nm avg. part. size, 5 mg/mL in toluene
Sigma-Aldrich
アフラトキシンM1, from Aspergillus flavus
Sigma-Aldrich
酸化鉄(II,III)、磁性ナノ粒子 溶液, 10 nm avg. part. size, 5 mg/mL in H2O
Sigma-Aldrich
酸化鉄(II,III)、磁性ナノ粒子 溶液, 5 nm avg. part. size, 5 mg/mL in H2O
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酸化鉄(II,III)、磁性ナノ粒子 溶液, 20 nm avg. part. size, 5 mg/mL in H2O
Sigma-Aldrich
酸化鉄(II,III)、磁性ナノ粒子 溶液, 5 nm avg. part. size, 5 mg/mL in toluene
Sigma-Aldrich
酸化鉄(II,III)、磁性ナノ粒子 溶液, 20 nm avg. part. size, 5 mg/mL in toluene
Supelco
アフラトキシンM1 溶液, 0.5 μg/mL in acetonitrile, analytical standard