コンテンツへスキップ
Merck
  • Lipase entrapment in protamine-induced bio-zirconia particles: characterization and application to the resolution of (R,S)-1-phenylethanol.

Lipase entrapment in protamine-induced bio-zirconia particles: characterization and application to the resolution of (R,S)-1-phenylethanol.

Enzyme and microbial technology (2012-05-15)
Jing-Yun Wang, Cui-Li Ma, Yong-Ming Bao, Pin-San Xu
要旨

Lipase from Burkholderia cepacia was encapsulated inside zirconia particles by biomimetic mineralization of K₂ZrF₆ induced with protamine, a natural cationic protein. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) were employed for the characterization of the novel immobilized lipase. SEM and TEM images showed that both the zirconia particles with and without lipase have good spherical structures with average particle sizes of 150 nm. Fluorescence microscopy demonstrated that the lipase was indeed encapsulated inside the zirconia particles. The maximum immobilization capacity of the zirconia particles was 0.15 units/mg under optimum immobilization conditions. Biochemical characterization showed that the encapsulated lipase could retain most of its initial activity. Compared with free lipase, the encapsulated lipase exhibited improved thermal, pH, and recycling stabilities. After 8 weeks of storage, no substantial loss in catalytic activity was observed for the encapsulated lipase. The conversion of the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate as acetyl donor catalyzed by zirconia-immobilized lipase reached 49.9% with higher ee(s) of 99.9% under the following optimal conditions: octane as solvent, 0.1M (R,S)-1-phenylethanol, 70 mg immobilized lipase, 180 rpm, 50 °C for 48 h. After 6 cycles (288 h), the conversion and ee(s) were still 43% and 85%, respectively.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
酢酸ビニル, contains 3-20 ppm hydroquinone as inhibitor, ≥99%
カタログ番号
容量
在庫状況
単価
数量
Supelco
酢酸ビニル, analytical standard
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
1-フェニルエタノール, 98%
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
α-メチルベンジルアルコール, ≥99%, FCC, FG
カタログ番号
容量
在庫状況
単価
数量
Supelco
酢酸ビニル 溶液, certified reference material, 5000 μg/mL in acetonitrile
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
酢酸ビニル, CP
カタログ番号
容量
在庫状況
単価
数量
酢酸ビニル, European Pharmacopoeia (EP) Reference Standard
カタログ番号
容量
在庫状況
単価
数量