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  • Bioaccessibility and cellular uptake by Caco-2 cells of carotenoids and chlorophylls from orange peels: A comparison between conventional and ionic liquid mediated extractions.

Bioaccessibility and cellular uptake by Caco-2 cells of carotenoids and chlorophylls from orange peels: A comparison between conventional and ionic liquid mediated extractions.

Food chemistry (2020-08-28)
Daniella C Murador, Leonardo M De Souza Mesquita, Bruna V Neves, Anna R C Braga, Paula L G Martins, Leila Q Zepka, Veridiana V De Rosso
要旨

Native extracts from orange peels were obtained by a conventional method using acetone and, an alternative method using ionic liquid (1-butyl-3-methylimidazolium chloride ([C4mim]Cl)). The bioaccessibilities and cellular uptakes of carotenoids, esters and chlorophylls were evaluated, since the influence of esterification on bioaccessibility and bioavailability is not well established. For this, the extracts were emulsified, submitted to in vitro simulated digestion model according to the INFOGEST protocol, followed by uptake by Caco-2 cells. Compounds were separated, identified and quantified by HPLC-PDA-MS/MS. After digestion, 22.0% and 26.2% of the total carotenoids and 45.9% and 68.7% of the chlorophylls were bioaccessible from the acetone and [C4mim]Cl extracts, respectively. The bioaccessibilities of xanthophylls and carotenes were significantly higher than those of the mono- and diesters. The uptake by Caco-2 cells varied from 130.2 to 131.9 ng/mg cell protein for total carotenoids and from 243.8 to 234.2 ng/mg cell protein for chlorophylls in the acetone and [C4mim]Cl extracts, respectively. In general, xanthophylls and esters were better absorbed than carotenes.

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製品内容

Sigma-Aldrich
ダルベッコ改変イーグル培地(高グルコース), With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
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ペニシリン-ストレプトマイシン, Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture
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ウシ胎児血清, non-USA origin, sterile-filtered, suitable for cell culture
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MEM非必須アミノ酸溶液 (100X), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
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ペプシン ブタ胃粘膜由来, powder, ≥250 units/mg solid
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α-アミラーゼ ブタ膵臓由来, Type VI-B, ≥5 units/mg solid
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リパーゼ ブタ膵臓由来, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
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パンクレアチン ブタ膵臓由来, 4 × USP specifications
Supelco
Amberlite XAD7HP, 20-60 mesh
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胆汁抽出物 ブタ