821892

ToyoScreen^® Phenyl-600M Process Development Column

phase phenyl, pkg of 6 × 1 mL, 65 μm particle size

• UNSPSCコード: 41115700

特性

• 物質: aluminum seal; polypropylene and polyethylene frit
• 製品種目: ToyoScreen^®
• アナライトの化学物質クラス: proteins
• 包装: pkg of 6 × 1 mL
• メーカー／製品名: Tosoh 21892
• パラメーター: ≤50% organic solvent; 0.2-1 mL/min flow rate; 3 bar max. pressure; 4 mL/min max. flow rate
• テクニック: HPLC: suitable
• L × 内径: 3 cm × 6.4 mm
• Matrix: hydroxylated methacrylic polymer
• マトリックス活性基: phenyl phase
• 粒径: 65 μm
• 使用pH: 1-13
• キャパシティ: 55 g/L±10 adsorption capacity (lysozyme)
• 分離法: hydrophobic interaction (HIC)

詳細

詳細

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen^® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl^® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

規格

Clean in place with 0.5 M NaOH or 0.1 M HCl.

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法的情報

ToyoScreen is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation