807573

TSKgel^® Phenyl-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, Phenyl phase

• 別名: TSKgel^® Hydrophobic Interaction Chromatography (HIC) Column
• UNSPSCコード: 41115700
• eCl@ss: 32110501

特性

• product name: TSK-GEL^®疎水性相互作用クロマトグラフィー(HIC)カラム, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
• 物質: PEEK
• 製品種目: TSKgel^®
• メーカー／製品名: Tosoh Phenyl-5PW, 07573
• パラメーター: ≤4 M salt concentration; ≤50% organic solvent; 1.2 mL/min flow rate; 10-50 °C temperature; 20 bar max. pressure
• テクニック: HPLC: suitable
• L × 内径: 7.5 cm × 7.5 mm
• マトリックス: polymer particle platform
• マトリックス活性基: Phenyl phase
• 粒径: 10 μm
• ポアサイズ: 1000 Å
• 使用pH: 2-12
• 分離法: hydrophobic interaction (HIC)

詳細

詳細

TSKgelフェニル-5PWは、3種類のTSK-GEL HICカラムの中で疎水性が最も高く、ほとんどの生体分子を選択性、保持率、および回収率で選別するための優れた選択肢です。1000Åの微細孔サイズを持つポリメタクリル酸系材料のフェニル-5PWカラムは、酸洗浄とアルカリ洗浄のどちらにも安定で、拡散係数の小さい大分子が容易に出入りします。TSKgel Phenyl-5PWではステンレス鋼が標準のカラム材質です。 ステンレス鋼に吸着する可能性のある試料のために、ガラスおよびPEEKのカラムハウジングが用意されています。一般用途向けには、これらに代わるカラム材質も適します。

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.

TSKgel BioAssist Phenyl columns contain the same particles as Phenyl-5PW columns, but are packed in PEEK column hardware rather than stainless steel. BioAsist Phenyl (Phenyl-5PW), TSKgel Ether-5PW and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing, such as BioAssist Phenyl. We recommend an Ether-5PW column for purifying very hydrophobic proteins. TSKgel BioAssist Phenyl and TSKgel Ether-5PW packings are based on TSKgel G5000PW resin - 10 μm particles with 1000 Å pores. TSKgel Butyl-NPR columns, which are less hydrophobic than Ether-5PW, is prepared from 2.5 μm nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

物理的形状

Shipped in water.

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法的情報

TSKgel is a registered trademark of Tosoh Corporation