Offering stock or providing custom endfittings for Supelco-manufactured HPLC columns is not available. The production process involves very tight specifications for the torque of the endfittings, and over or under tightening can significantly affect performance.
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製品名
Ascentis® C18 HPLCカラム, 5 μm particle size, L × I.D. 25 cm × 10 mm
物質
stainless steel column
品質水準
認証
suitable for USP L1 (Similar to Phenomenex Luna C18)
製品種目
Ascentis®
特徴
endcapped
メーカー／製品名
Ascentis®
包装
1 ea of
標識化の程度
25% Carbon loading
パラメーター
≤70 °C temp. range
400 bar pressure (5801 psi)
テクニック
HPLC: suitable
L × 内径
25 cm × 10 mm
表面積
450 m2/g
表面被覆率
3.7 μmol/m2
不純物
<5 ppm metals
Matrix
fully porous particle
silica gel high purity, spherical
マトリックス活性基
C18 (octadecyl) phase
粒径
5 μm
ポアサイズ
100 Å
動作pH範囲
2-8
アプリケーション
food and beverages
分離法
reversed phase
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関連するカテゴリー
詳細
AscentisカラムファミリーはSupelcoの科学者達が開発した第4世代のHPLCカラム技術です。Ascentisは孔径100Åの高純度シリカ(粒径は3、5、10 μm)に官能基を結合させています。カラムは低分子用として設計されており、マイクロカラム(内径1.0 mm)から調製用(内径50 mm)まで揃っています。ファミリーにはC18、C8、Phenyl、Siに加えて、極性基導入型であるRP-Amideがあります。
Ascentis C18は、検出しにくい化合物に対しても、対称的なピーク形状と優れた保持時間を示す、非常に安定した信頼性の高いHPLCカラムの第一候補です。
Ascentis C18は、検出しにくい化合物に対しても、対称的なピーク形状と優れた保持時間を示す、非常に安定した信頼性の高いHPLCカラムの第一候補です。
アプリケーション
- A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals: This paper details the application of Ascentis® C18 columns in a clinical setting to measure cortisol levels in urine. The method provides a fast, accurate, and reliable way to assess patient hormonal profiles, critical for diagnosing endocrine disorders (Zhao et al., 2022).
- A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS: Utilizing Ascentis® C18, this study compares different analytical methods for quantifying ginsenosides. It showcases the column′s adaptability and precision in natural product research, essential for ensuring the quality and efficacy of herbal medicines (Hsu et al., 2022).
- Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Highlighting the Ascentis® C18′s effectiveness in pharmaceutical formulations, this research demonstrates its capacity to analyze complex drug delivery systems, contributing to the development of more effective therapeutic solutions (Brugnera et al., 2022).
- Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method: This publication illustrates the application of the Ascentis® C18 in veterinary medicine, providing crucial insights into drug stability and composition which are vital for regulatory compliance and animal safety (Padivitage et al., 2022).
特徴および利点
- 優れた保持率
- 対称性高いピ-ク形状
- 高い再現性
- 完璧なLC-MS適合性
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
581343-U:
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Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
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Is it possible to obtain a new HPLC column end fitting for the Ascentis C18 column with a 10mm ID, product number 581343-U?
1 回答-
役に立ちましたか？
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What does the size 10.32 refer to for HPLC fittings?
1 回答-
The size 10-32 for HPLC fittings refers to a male nut with a 10 (.190") diameter thread barrel and 32 threads per inch, indicating a narrower nut with a denser thread count.
役に立ちましたか？
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アクティブなフィルタ
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