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資料

原産地証明書（COO）/試験成績書（COA）

567532-U

Discovery^® HS F5 (3 µm) HPLC Columns

Name: Discovery<SUP>&#174;</SUP> HS F5 (3 µm) HPLC Columns
Brand: SUPELCO

L × I.D. 15 cm × 4 mm, HPLC Column

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About This Item

UNSPSCコード:

41115700

eCl@ss:

32110501

NACRES:

SB.52

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Discovery^® HS F5 HPLCカラム

product name

Discovery^® HS F5 HPLCカラム, 3 μm particle size, L × I.D. 15 cm × 4 mm

物質

stainless steel column

認証

suitable for USP L43

製品種目

Discovery^®

特徴

endcapped

メーカー／製品名

Discovery^®

包装

1 ea of

標識化の程度

12% Carbon loading

パラメーター

≤70 °C temp. range
400 bar pressure (5801 psi)

テクニック

HPLC: suitable
LC/MS: suitable

L × 内径

15 cm × 4 mm

表面積

300 m²/g

表面被覆率

4 μmol/m²

不純物

<10 ppm metals

マトリックス

silica gel, high purity, spherical particle platform
fully porous particle

マトリックス活性基

PFP (pentafluorophenyl) phase

粒径

3 μm

ポアサイズ

120 Å

operating pH range

2-8

アプリケーション

food and beverages

分離法

reversed phase

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詳細

C18カラムでの方法をDiscovery® HS F5 HPLCカラムに移行するためのガイドライン：
塩基性物質は通常C18カラムよりもHS F5カラムのほうが長く保持されます。C18カラムでの分離における有機溶媒の濃度を5～10％上昇させると、通常HS F5カラムで類似した保持が再現されます。他の化合物での結果は大きく異なります。しかし、2.5未満のlog P_o/w値の溶質はC18と比べてHS F5 では通常長く保持されます。差の程度は溶質に大きく依存しています。

Discovery® HS F5の結合相は、C18カラムとは極めて異なった逆相分離を提供します。しかし、化合物は通常同じ保持時間枠内に溶出されるので、多くの場合C18カラムでの方法を容易に移行できます。

特徴および利点

独自の選択性
C18と同等の保持(強い移動相が必要な場合もあります)
優れたピ-ク形状
安定で、低ブリ-ドのLC-MS分離
粒径3~10 μmで、スケ-ルアップ可能な分離

法的情報

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

ガードカートリッジ

製品番号

詳細

価格

567573-U

Discovery^® HS F5スペルガードカートリッジ, 3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit

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"Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.

Umberto M Battisti et al.

Journal of chromatography. A, 1443, 152-161 (2016-03-30)

A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.

HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).

Jennie Wang et al.

Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)

An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic

Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.

Lucie Havlíková et al.

Talanta, 76(3), 597-601 (2008-07-01)

A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using

High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.

Jana Maláková et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)

Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation

Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase.

Federica Pellati et al.

Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)

In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an

資料

Discovery^® HS F5 (3 µm) HPLC Columns