TRUE6

TrueGel3D Hydrogel Kits

SLO-DEXTRAN, allow cell recovery, PEG non cell-degradable crosslinker

• 別名: 3D Hydrogel Kit, TrueGel3D Kit
• UNSPSCコード: 12352207
• NACRES: NA.71

特性

• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-DEXTRAN. The polymer, SLO-DEXTRAN is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-DEXTRAN. SLO-DEXTRAN does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

アプリケーション

TRUE6-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic natural tissue stiffness and to investigate the effect of mechanical stimulus on cell physiology and behavior. It is also used to culture non-proliferating cells and proliferating spheroids that do not attach to hydrogel for survival, but rather use their own extracellular matrix.

特徴および利点

• Slow gelling (takes 8 minutes to 25 minutes, depends on gel stiffness)
• Flexibility in modulating gel stiffness
• Does not allow cell migration
• Allow cell recovery and post-culture analyses
• Cell inert hydrogel (if not customized with RGD peptide)

構成

TRUE6-1KT contains SLO-DEXTRAN solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

注意

Avoid prolong exposure of PEG non cell-degradable crosslinker to air.

安全性情報

• ピクトグラム: GHS05
• シグナルワード: Warning
• 危険有害性情報: H290,H315,H319
• 注意書き: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• 危険有害性の分類: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• 保管分類コード: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3