T6454

Anti-Timeless (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: 抗TIM抗体
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~160 kDa
• 交差性: human
• 包装: antibody small pack of 25 μL
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): 5-10 μg using HEK-293T cells over expressing human Timeless; indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human Timeless; western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing human Timeless
• UniProtアクセッション番号: Q9UNS1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TIMELESS(8914)

詳細

詳細

The gene TIMELESS is located on the human chromosome at 12q13.3. The encoded protein is a component of the replisome complex.

特異性

Anti-Timeless (C-terminal) recognizes human Timeless (also known as hTIM, TIM, TIM1, Timelesss1).

アプリケーション

Anti-Timeless (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

生物化学的／生理学的作用

Timeless (Tim) forms a complex with Tim interacting protein (Tipin), found by two-hybrid screening. It associates with components of the replication fork complex through direct binding with the (RPA) and are important for efficient DNA synthesis. Timeless plays a role in replication checkpoint responses to genotoxic stress. It also plays an important role in the maintenance of genome stability during unperturbed DNA replication. Overexpression of the TIMELESS gene is associated with cervical cancer. Annihilation of this gene induces apoptosis and cell senescence.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)