SRP4858

熱ショックタンパク質90 ヒト

recombinant, expressed in baculovirus infected Sf9 cells, ≥99% (SDS-PAGE)

• 別名: HSP86, HSPC1, HSPCA, HSPN, Hsp89, LAP2
• MDL番号: MFCD02253027
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: ≥99% (SDS-PAGE)
• フォーム: liquid
• 分子量: ~90 kDa
• 包装: pkg of 25 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 不純物: endotoxin, tested
• NCBIアクセッション番号: P08238
• UniProtアクセッション番号: P14625
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... HSP90B1(7184)

詳細

詳細

Heat shock protein 90 (HSP90) is a molecular chaperone and one of the most abundant proteins in unstressed cells.[1] In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90α showing 85% sequence identity to Hsp90β. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90β subunit.

生物化学的／生理学的作用

Heat shock protein 90kDa beta member 1 (HSP90B1) is activated during apoptosis. It is associated with the endoplasmic reticulum membrane and undergoes specific proteolytic cleavage leading to the activation of the caspase CPP32 and initiation of DNA fragmentation. The protein protects cells against flux in Ca²⁺ homeostasis, which would otherwise result in cell death. It maintains the chromosomal stability in mammalian cells by associating with FAC. HSP90B1 chaperones toll-like receptors (TLRs) and thus optimizes the B cell functioning. These proteins are also the main downstream chaperones which mediate the ER unfolded protein response (UPR), which is very essential for maintaining protein homeostasis in the ER.[1][2][3]

物理的形状

タンパク質溶液(40 mM HEPES/KOH、pH 7.5、400 mM塩化カリウム、5%グリセロール含有)

調製ノート

バイアルは、開封前に遠心してください。凍結融解の繰り返しは避けてください。

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable