SRP0717

NEK8, Active

PRECISIO^® Kinase, recombinant

• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: 70-95% (SDS-PAGE)
• フォーム: frozen liquid
• 比活性: 5.0-7.0 nmol/min·mg
• 分子量: ~66 kDa
• 濃度: 0.05 μg/μL
• アクセッション番号: NM_178170
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... NEK8(284086)

詳細

アプリケーション

Useful for kinase assay and Western blot.

生物化学的／生理学的作用

Recombinant human NEK8 (1-322) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK8 or NIMA-related kinase 8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans which may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development ⁽¹⁾. NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins ⁽²⁾.

再構成

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

保管および安定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法的情報

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable