SRP0119

Sirtuin 5 human

recombinant, expressed in E. coli, ≥50% (SDS-PAGE)

• 別名: NAD-dependent ADP-ribosyltransferase sirtuin-5, SIR2L5, SIRT5, sir2-like 5
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥50% (SDS-PAGE)
• フォーム: aqueous solution
• 分子量: 59.8 kDa
• 包装: pkg of 100 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_012241
• UniProtアクセッション番号: Q9NXA8
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... SIRT5(23408)

詳細

詳細

The sirtuin 5 (SIRT5) gene with eight exons is mapped to human chromosome 6p23. The gene exists in two isoforms codings for 310 amino acid and 299 amino acid protein, respectively. Sirtuin 5 is mainly present in heart muscle cells and in lymphoblasts. It is a member of the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (HDACs).[1] Sirtuin 5 is a mitochondrial protein.[2]
Human Sirtuin 5, GenBank Accession No. NM_012241), full length with N-terminal ST tag, MW = 59.8kDa, expressed in Escherichia coli expression system.

アプリケーション

酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。

生物化学的／生理学的作用

Sirtuin 5 (SIRT5) catalyzes the degradation of negatively charged acylated substrates. It also possesses desuccinylase, demalonylase and deglutarylase activities.[2] SIRT5 is a nicotinamide adenine dinucleotide (NAD⁺)-dependent deacetylase and is overexpressed in non-small cell lung cancer (NSCLC) and may have a role in cancer growth.[1][3] Members of sirtun family play a vital role in epigenetic gene silencing, DNA repair and recombination, cell-cycle, microtubule organization, and in the regulation of aging.[1] SIRT5 has been involved in regulation of the carbamoyl phosphase synthase 1 activity, an essential enzyme of urea cycle, via maintaining lysine glutarylation levels. Sirtuin 5 as a mitochondrial protein plays a major role in controlling ATP production, apoptosis, and cell signaling.[4]

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable